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. 2022 Nov 8;12:19009. doi: 10.1038/s41598-022-23328-0

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Workflow for the OPNested, OPNested + GMM, and OPNested − GMM ComBat implementations for sequential harmonization given two batch effects. Red denotes a batch effect, while the dash indicates that the data has been harmonized by a particular batch effect (i.e., Data-1 means the data has been harmonized by batch effect 1, Data-1-2 means Data-1 has been harmonized again by batch effect 2, etc.).

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