Figure 1.

CaMKII peptide (CQRSTVASMMHRQETVD) oxidized by H₂O₂forms a disulfide-linked dimeric peptide.A, general structure of a CaMKII monomer. The amino acid sequence of the regulatory domain is highlighted. B, calculated rate of Met or (C) Met and Cys oxidized by H₂O₂. D, chromatogram and measured mass of the native CaMKII peptide using HPLC-MS/MS (representative image of n = 2 replicates). E, CaMKII peptide treated with H₂O₂ (representative image of n = 2 replicates). F, CaMKII peptide treated with H₂O₂ and DTT (representative image of n = 2 replicates). CaMKII, calcium/calmodulin-dependent protein kinase II; mAU, milliabsorbance units.