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. 2022 Oct 8;298(11):102579. doi: 10.1016/j.jbc.2022.102579

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Cys273 oxidation is sufficient to induce CaMKII autonomy in an HEK cell lysate.A, total activity in lysates of CaMKII Cys273-Cys290, Cys273, and Cys290 mutants expressed in HEK cells (without EGTA) compared to nontransfected HEK cells (NT). The activity of Cys273-Cys290 was set to 100%. n = 3 replicates. B, autonomous activity in lysates of NT, Cys273-Cys290, Cys273, and Cys290 mutants expressed in HEK cells with the lysate incubated or not with diamide, in the presence or not of thioredoxin. n = 4 replicates. ∗p < 0.05 compared with CYs273-Cys290 by unpaired t test. CaMKII, calcium/calmodulin-dependent protein kinase II.