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. 2022 Oct 8;298(11):102579. doi: 10.1016/j.jbc.2022.102579

Figure 6.

Figure 6

MMVV mutant is degraded faster than the WT enzyme.A, purified WT and MMVV CaMKII mutants' total activity (no EGTA addition). N = 3 replicates. Normalized to WT (100%). B, autonomous activity of WT and MMVV CaMKII mutants treated with diamide (after EGTA addition). N = 3 replicates. % of total activity (no EGTA addition). ∗∗p = 0.04 compared with WT by unpaired t test. C, HEK293T cells transfected with WT or MMVV CaMKII mutants were treated with 50 μg/ml cycloheximide (CHX). Cells were lysed and the supernatant probed with anti-CaMKII antibody. Representative image of n = 3 replicates. D, half-life of CaMKII WT (blue) or MMVV mutant (orange). The level of CaMKII at 0 h was set to 100%. Protein loading was normalized by total protein staining. ∗∗p = 0.021 compared with WT by unpaired t test. Fit to first order decay by Prism. CaMKII, calcium/calmodulin-dependent protein kinase II.