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. 2022 Nov 8;13:6732. doi: 10.1038/s41467-022-34612-y

Fig. 1. Overall structure of the EARS1–IARS1 complex.

Fig. 1

a A list of MSC components identified by mass spectrometry. Total spectrum count of identified proteins interacting with IARS1ΔC, IARS1 UNE-I, or IARS1 WT is shown. b Affinity pull-down analysis of the interaction between EARS1 and IARS1. The input (I) and eluate (E) fractions were analyzed by SDS-PAGE and Coomassie Blue staining. Black and red arrowheads indicate eluted His-IARS1 and GST-EARS1, respectively. The data are representative of three independent experiments. Source data are provided as a Source Data file. c A schematic illustration of the architectures of EARS1 and IARS1. d Structure of the EARS1–IARS1 complex. The core regions of EARS1 (light blue) and IARS1 UNE-I (KH, green; UBX1, orange; and UBX2, pink) are shown at the top and bottom of the image, respectively. Close-up views of the boxed regions are shown in Fig. 2a–c. e–g Structures of UBX1 e, UBX2 f, and Ub g.