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. Author manuscript; available in PMC: 2023 Nov 15.
Published in final edited form as: J Immunol. 2022 Nov 15;209(10):1950–1959. doi: 10.4049/jimmunol.2200495

Figure 1: Effect of E6020 in activating macrophages from TLR4-SNP mice.

Figure 1:

Thioglycollate-elicited peritoneal macrophages were harvested from age-matched C57BL/6J, TLR4-SNP, and TLR4−/− mice and stimulated with increasing concentrations of E6020 (0.1 ng/ml-100 ng/ml) for 2 hours, and RNA was processed as described in Methods. Gene expression of Il1b (A), Tnf (B), Ifnb1 (C) and Cxcl10 (D) were determined. Data were pooled from 4 independent experiments with cells pooled from 3 mice/genotype/experiment and presented as -ΔCT (left y-axis) and fold induction (2^-ΔΔ CT value; right y-axis), mean +/− SEM. Statistical analysis (Repeated measures 2-Way ANOVA with Sidak’s post-hoc comparison) was performed on the average -ΔCT values of WT and TLR4-SNP macrophage responses from each experiment.