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. Author manuscript; available in PMC: 2023 Nov 15.
Published in final edited form as: J Immunol. 2022 Nov 15;209(10):1960–1972. doi: 10.4049/jimmunol.2200543

Figure 1. Conditional deletion of Ncf2 and Cybb NOX2 subunits and characterization of expression and NOX2 activity in phagocytes.

Figure 1.

(A) Dihydrorhodamine (DHR) assay for NOX2 activity in peripheral blood PMN. Percent of DHR-high, low DHR-low and DHR-negative cells as indicated in Ncf2fl/fl, Ncf2KO, Ncf2LysMcre, Ncf2S100A8Cre, CybbS100A8Cre and Ncf2CybbS100A8Cre mice. Representative samples shown. (B, C) Superoxide production by cytochrome C assay of marrow neutrophils from Ncf2fl/fl, Ncf2LysMcre and Ncf2S100A8Cre mice (B) and Ncf2fl/fl, Ncf2S100A8Cre and Ncf2CybbS100A8Cre mice (C). (D) Percent of oxidase positive (NBT-positive) RPM and AM in the indicated mice. 200 cells scored per mouse. (E) Monocyte stimulation index calculated from DHR+ bone marrow monocytes in the indicated mice. Each data point represents one mouse with n≥4 per group. Bar graph data shown as mean ± SD. Student’s ‘t’ test was performed for comparisons between 2 groups and **P<0.01, ***P<0.001 ****P< 0.0001 were considered as significant. Data represents at least 2 independent sets of experiments.