Figure 5. Reduced neutrophil NOX2 activity impairs control of A. fumigatus in corticosteroid-treated mice.
a) Schema for AF293 challenge of cortisone acetate-treated Ncf2fl/fl, Ncf2LysMCre, and Ncf2S100A8Cre mice, with analysis at 7 days after challenge. B) Total leukocyte and neutrophil counts from 1 ml BAL fluid. (C) Aspergillus CFU in homogenate of right lung for the indicated mice. (D) Representative histology of lung sections for the indicated genotypes. From left to right, panels show H&E (bar 1 mm), and GMS (bar 100 μm). Arrows indicate hyphae.
Data represents 3 independent sets of experiments with n≥5 per group. Graphical data shown as mean ± SD. Student’s ‘t’ test was performed for comparisons between 2 groups and *P<0.05, **P<0.01, ***P<0.001 ****P< 0.0001 were considered as significant.