Figure 6. Sterile inflammation is increased in conditional NOX2-deleted mice and differentially dependent on neutrophil or macrophage NOX2 activity.

(A) Schema for zymosan-induced lung inflammation in indicated mice. Mice were challenged with 20 μg zymosan IN and studied after 18 hours. (B) Total leukocyte counts from 3 ml BAL fluid. The percentage of neutrophils were identified by cytospin and used to calculate total BAL neutrophils. (C) Representative histology of H&E-stained lung sections for the indicated genotypes. Scale bar for top row of panels is 500 μm and 100 μm for the lower panels. Arrows indicate neutrophil foci. (D) LTB4 ELISA from supernatants obtained from purified bone marrow neutrophils incubated for one hour either with no stimulus (NS) or with zymosan (1 neutrophil:2 zymosan particles). Ncf2^fl/fl, Ncf2^S100A8Cre and Ncf2^KO mice were studied as indicated. (E) Schema for periodate-induced peritonitis in indicated mice and (F) peritoneal lavage cell counts and % neutrophils at 72 hours following IP injection of periodate. Data represents 3 independent sets of experiments with n≥4 per group. Graphical data shown as mean ± SD. Student’s ‘t’ test was performed for comparisons between 2 groups and *P<0.05, **P<0.01, ***P<0.001 ****P< 0.0001 were considered as significant.