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. Author manuscript; available in PMC: 2023 Nov 9.
Published before final editing as: Ann Rheum Dis. 2022 May 9:annrheumdis-2021-221867. doi: 10.1136/annrheumdis-2021-221867

Figure 3. Regulation of chondrogenic and anabolic genes by KLF4 and KLF2 in human OA chondrocytes, meniscal cells and BMSC pellets.

Figure 3.

(A and B) Human OA chondrocytes were transfected with adenovirus (Ad-KLF4 or Ad-EGFP for (A), and Ad-KLF2 or Ad-EGFP for (B)), and RNA was collected 48 hours after transfection (n=9 donors). (C and D) Human meniscal cells were transfected with adenovirus (Ad-KLF4 or Ad-EGFP for (C), and Ad-KLF2 or Ad-EGFP for (D)), and RNA was collected 48 hours after transfection (n=4 donors). (E and F) Human bone marrow-derived mesenchymal stem cells (BMSCs) were transfected with adenovirus (Ad-KLF4 or Ad-EGFP for (E), and Ad-KLF2 or Ad-EGFP for (F)), and were cultured in pellets. RNA was collected 2 weeks after pellet culture (n=3 donors). mRNA levels are expressed as means±SE, relative to Ad-EGFP. *P<0.05, **P<0.01, paired t-test.