Table 3.
Summarized details of the selected aptamers for POCT
| POCT platform | Target | Aptamer selection method | Name of aptamer (type) | Sequence (from 5′ to 3′) | Ref |
|---|---|---|---|---|---|
| Mask-based electrochemical platform | SARS-CoV-2 | S1 protein-based SELEX | CFA0688T (DNA) | BasePairBio Inc | [158] |
| Nanochannel-based electrochemical platform | SARS-CoV-2 | S1 protein-based SELEX | XN-268 s (DNA) | CACGCATAACCGAGCTGGGGTGGGGTAGTGGTATGGAGCGTCAGTTGTTATGCGTG | [159] |
| Microelectrode array chip-based platform | SARS-CoV-2 | N protein-based SELEX | A48 (DNA) | GCTGGATGTCGCTTACGACAATATTCCTTAGGGGCACCGCTACATTGACACATCCAGC | [160] |
| Gold interdigitated electrode-based platform | SARS-CoV-2 | N protein-based SELEX | NP aptamer (DNA) | GCAATGGTACGGTACTTCCGGATGCGGAAACTGGCTAATTGGTGAGGCTGGGGCGGT | [161] |
| Glucometer-based platform | SARS-CoV-2 | N protein-based SELEX | NP aptamer (DNA) | GCAATGGTACGGTACTTCCGGATGCGGAAACTGGCTAATTGGT GAGGCTGGGGCGGT | [162] |
| Lateral flow assay-based platform | C-reactive protein | Microfluidic technology-assisted SELEX | Apt1 (DNA) | GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT | [177] |
| Smartphone-based platform | Streptomycin | SELEX | APT (DNA) | TAGGGAATTCGTCGACGGATCCGGGGTCTGGTGTTCTGCTTTGTTCTGTCGGGTCGTCTGCAGGTCGACGCATGCGCCG | [179] |
| Smartphone-based platform | Mycotoxin (ochratoxin A) | SELEX | 1.12.2 (DNA) | GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA | [180] |
| Pressuremeter-based platform; microfluidic paper-based platform | Cocaine and ochratoxin A | SELEX | Aptamer (DNA) | GGGAGACAAGGATAAATCCTTCAATGAAGTGGGTCTCCC (cocaine); GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA (ochratoxin A) | [183, 184] |
| Multiplexed bar-chart chip-based platform | S. enterica, E. coli, and L. monocytogenes | Cell-SELEX | Aptamer (DNA) | TATGGCGGCGTCACC CGACGGGGACTTGACATTATGACAG (S. enterica); CCGGACGCTTATGCC TTGCCATCTACAGAG CAGGTGTGACGG (E. coli); TACTATCGCGGAGACAGCGCGGGAGGCACCGGGGA (L. monocytogenes) | [185] |