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. 2022 Oct 26;13:1025377. doi: 10.3389/fimmu.2022.1025377

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Copyright © 2022 Chunder, Schropp, Jabari, Marzin, Amor and Kuerten

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of activation markers and survival of B cells after MMP-3 stimulation. (A) Expression of CD69, CD80 and CD86 and viability of unstimulated (cells isolated from n = 4 – 6 healthy donors) B cells after treatment with APMA-activated rMMP-3 compared to APMA (vehicle) only. (B) Expression of CD69, CD80 and CD86 and viability of pre-stimulated (cells isolated from n = 5-7 healthy donors) B cells incubated with APMA-activated rMMP3 or APMA (vehicle) only. Relative expression of CD69, CD80 and CD86 and viability of either unstimulated (A) or pre-stimulated B cells (B) after treatment with rMMP-3 compared to vehicle is also shown. Graphs show mean values + SEM. *p < 0.05, **p < 0.01, ***p < 0.001; paired t test, Wilcoxon test. APMA, 4-aminophenylmercuric acetate.