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. 2022 Oct 26;13:1025377. doi: 10.3389/fimmu.2022.1025377

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Copyright © 2022 Chunder, Schropp, Jabari, Marzin, Amor and Kuerten

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine profiling of MMP3-treated B cells. (A) Layout of the antibody array used for investigating cytokines. (B) The dot blot indicates the cytokine expression profile of pre-stimulated B cells after treatment with either MMP-3 or vehicle from one representative donor. Additionally, IL-6 production by B cells from the same donor is highlighted. (C) Relative amount of IL-6 production by pre-stimulated B cells treated with MMP-3 compared to vehicle was calculated by using integrated density from n = 1 donor, measured in duplicates. (D) Relative IL-6 production of pre-stimulated B cells (n = 9 healthy donors), measured by ELISA. Bars display mean values + SEM, **p < 0.01, paired t test. Pos, positive control; Neg, negative control.