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. 2022 Oct 20;13:1002565. doi: 10.3389/fphar.2022.1002565

FIGURE 2.

FIGURE 2

High METTL3 expression is associated with proliferation, apoptosis, migration and invasion in ESCC cells (A). METTL3 mRNA and protein levels were drastically elevated following lentiviral transfection of KYSE510 and KYSE30 cells. (B). The CCK-8 assay assessed the proliferation ability of METTL3-overexpressing KYSE510 and KYSE30 cells. (C). Flow cytometric analysis was conducted to assess apoptosis in KYSE510 METTL3-overexpressing cells. (D). The clonality of KYSE510 and KYSE30 cells was measured by the plate clone. (E). The wound scratch test and (F). Transwell migration and invasion assays were conducted to examine the migratory and invasion capacities of KYSE510 and KYSE30 cells overexpressing METTL3. (G). Expression of E-cadherin, MMP19 and Vimentin proteins was detected via western transfer in METTL3-overexpressing cells. The protein levels of Bcl2, Caspase 3 and Cyclin D1 were detected by western blotting in METTL3-overexpressing cells. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.