FIGURE 5.

IFIT2 overexpression inhibited ESCC cell proliferation and invasion (A, B). To evaluate the effectiveness of the IFIT2 overexpression lentivirus, qRT‒PCR was used to detect mRNA levels, and western blot analyses were used to detect protein levels. (C). A CCK-8 assay was used to evaluate the proliferation ability of KYSE510 and KYSE30 cells after IFIT2 overexpression. (D). Cell proliferation capabilities were identified through colony formation tests in transfected KYSE510 and KYSE30 cells. (E). The wound healing assay reduced the KYSE510 and KYSE30 cell ability to migrate. (F). Transwell assays were used to analyze transfected cell migration and invasion. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.