FIGURE 4.

Localization of CMV‐3a‐GFP and mRFP‐RTN3 in stably transformed Arabidopsis cells. Coexpression of the viral movement protein CMV‐3a‐GFP and the reticulon protein mRFP‐RTN3 results in a change of subcellular localization for one of the proteins. Mainly, CMV‐3a‐GFP is no longer restricted to plasmodesmata (a) but also labels the endoplasmic reticulum in puncta (b). In rarer cases, mRFP‐RTN3 no longer labels the whole endoplasmic reticulum (c) but is restricted to plasmodesmata (d). Scale bars = 5 μm. See Figure S4 for a more detailed presentation of protein localizations at the cell surface and median.