Fig. 1.

Kdm6b is upregulated upon neural commitment. a Map of the mouse Kdm6b locus showing the CpG islands (CGI) and exons as described in the UCSC Genome Browser. Positions are given according to the mm9 assembly. b Kdm6b expression level in ES cells (n = 6) and at day 4 (D4; n = 3), D8 (n = 3), and D12 (NP cell stage; n = 6) of in vitro corticogenesis. The expression was assessed with primers located on exons 5 and 6. Statistical significance was determined by one-way ANOVA (comparison of each differentiation point with ES cells). c KDM6B protein level evaluated by western blotting in ES and NP cells (D12). d ChIP analysis of H3K9ac, H3K4me3, H3K27me3, and H3K9me3 enrichment at CGI1 and CGI2 in ES (n = 3) and NP (n = 3) cells. The precipitation level was normalized to that obtained at the Rpl30 (for H3K4me3 and H3K9ac), HoxA3 (for H3K27me3), and IAP (for H3K9me3) promoters. Statistical significance was determined with the unpaired t test (p values in the figure). The data are presented as the mean ± SEM