Fig. 7.

Inhibition of miR-150-5p in BMSC-exosomes offsets the therapeutic effects of BMSC-exosomes on mechanical allodynia. A The transfection efficiency was observed under a fluorescence microscope; B RT-qPCR was used to detect the expression of miR-150-5p in BMSCs and BMSC-exosomes. RT-qPCR C and western blotting D were used to detect the expression of miR-150-5p and NOTCH2 in the SDH of SNL rats; Hind paw PWT E and PWL F of SNL rats were measured on days 0, 1, 3, 5, 10, 15, and 20 after surgery; G TUNEL staining revealed the apoptosis in the SDH; H ELISA detected the expression levels of TNF-α, IL-1β and IL-6 in the SDH. Data were shown as mean ± standard deviation. N = 6. ^&P < 0.05, vs. the vector or Exo-vector group. BMSC bone marrow mesenchymal stem cell, SNL spinal nerve ligation, SDH spinal dorsal horn, PWT paw withdrawal threshold, PWL paw withdrawal latency