FIGURE 3.

Heatmap of the phenotypic characterization of the main multi-drug resistance profiles (MDRPs) identified by the Kirby-Bauer Disk Diffusion Susceptibility Test in randomly selected transconjugants. A total of 28 different phenotypic MDRPs were transferred from bacterial communities from the two sites in the La Paz River basin to E. coli CV601 recipients. A single profile was defined as the unique combination of susceptibility, intermediate resistance, or resistance to the 13 antibiotics included in the analysis. Color code: black = susceptible (S), magenta = intermediate (I), and yellow = resistant (R). Antibiotics: AMP, ampicillin; TET, tetracycline; CIP, ciprofloxacin; CHL, chloramphenicol; CTX, cefotaxime; MEM, meropenem; DOR, doripenem; ETP, ertapenem; IMI, imipenem; NA, nalidixic acid; CN, gentamicin; STR, streptomycin; PTZ, piperacillin-tazobactam; SMX/TMP, sulfamethoxazole/trimethoprim. All MDRPs include resistance to SMX/TMP because all transconjugants were selected from conjugation experiments using plates supplemented with this specific combination of antibiotics. Correlation was used as the clustering method. The pheatmap R program was used to generate the heatmap.