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SDS-PAGE and Western immunoblot analysis of whole cells of parent strain NG8 (lanes 1) and spaP mutant strain PC3370 (lanes 2) boiled in nonreducing SDS sample buffer. After electrophoresis, proteins were stained with Coomassie brilliant blue R-250 (A) or transferred to nitrocellulose membranes and reacted with a 1:1,000 dilution of anti-P1 monoclonal antibody 4-10A8c (B).
