FIG. 3.

Immunoblot with antisera to α₁PI in which gel samples were comprised of 10 μg of α₁PI plus 200 μg of trypsin which had been incubated for 30 min at 37°C. Lanes 1 to 3 show the results obtained when α₁PI was previously exposed to 6 mM NADH and 100 μM pyocyanin alone (lane 1), or with SOD (300 U/ml; lane 2) or catalase (5,000 U/ml; lane 3) added, for 20 min prior to the addition of trypsin to the reaction mixture. Samples in lanes 4 to 6 were obtained under the same conditions as those of lanes 1 to 3, except that the trypsin rather than the α₁PI was previously exposed to 6 mM NADH and 100 μM pyocyanin alone (lane 4), or with SOD (300 U/ml; lane 5) or catalase (5,000 U/ml; lane 6) added, for 30 min prior to its interaction with α₁PI. The results are representative of three experiments.