FIG. 7.

Immunoblot with antisera to α₁PI in which gel samples were comprised of 10 μg of α₁PI alone (lane 1) or 10 μg of α₁PI alone plus 200 μg of trypsin (lane 2), which had been incubated for 30 min at 37°C. The reaction in lane 3 was performed under the same conditions as those of lane 2, except that the α₁PI had been exposed to 14 mM β-mercaptoethanol prior to the addition of the trypsin. Although not shown in this figure, addition of β-mercaptoethanol after the completion of the incubation of trypsin and α₁PI has no effect on complex formation.