FIG 5.

Analysis of expression of 62 autophagy genes in macrophages isolated from transgenic mice. BM cells from WT, M1-γ34.5, and M2-γ34.5 mice were isolated, and macrophages were generated and infected with 10 PFU/cell of HSV-1 strain McKrae. Total RNA was isolated from infected macrophages and used for NanoString analysis of 62 autophagy genes. The autophagy panel of 62 genes is illustrated in a pie chart showing the autophagy genes commonly expressed in WT, M1-γ34.5, and M2-γ34.5 groups (significant common genes), uniquely expressed in M1-γ34.5 macrophages, M2-γ34.5 macrophages, or WT macrophages, or commonly expressed in 2 of 3 groups and autophagy genes whose expression did not differ significantly from mock (nonsignificant genes). Gene expression analysis is based on three replications.