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ITCH forms a complex with bat hepatovirus pX. (A) Global Needleman-Wunch amino acid sequence alignment of pX from human hepatovirus A virus (HAV; HM175 virus; GenBank accession no. NP_041007) and M32 virus, which was recovered from the African straw-colored fruit bat Eidolon helvum (hepatovirus H; GenPept accession no. ALL35271). (B) Coimmunoprecipitation of EPN-M32pX, EPN-pX, and EPN-PAD from lysates of transfected 293T cells.
