FIG 6.

Effect of HBc overexpression or reconstitution on cccDNA transcription in vivo. (A) AAV-HBV1.04-WT was cotransduced into C57BL/6 mice with AAV-GFP or AAV-HBc at 1 × 10¹¹ viral genome (vg) equivalents through tail vein injection, and samples were collected at the indicated time points. (B) Liver samples were collected and subjected to Western blotting for cellular HBsAg and capsid detection. (C) The level of HBsAg in mouse serum was tested by ELISA after 1:1,000 dilution. (D) AAV-HBV1.04-Δcore was cotransduced into C57BL/6 mice with AAV-GFP or AAV-HBc at 1 × 10¹¹ viral genome (vg) equivalents through tail vein injection, and samples were collected at the indicated time points. (E) The expression of HBsAg and capsid was detected by Western blotting. (F) The level of HBsAg in mouse serum was evaluated by ELISA after 1:1,000 dilution. Data were presented as mean ± SD.