TABLE 2.
Antibodies
| Antibody | Company/reference | Catalog no. | Host | Dilution(s) |
|---|---|---|---|---|
| HBc | Self-made (18) | Rabbit | 1:1,000 (Western blot); 1:20 (ChIP assay) | |
| HBc | Gene Technology | GB058629 | Rabbit | 1:50 (immunostaining) |
| HBsAg | Novus Biologicals | NB100-62652 | Rabbit | 1:2,000 (Western blot) |
| HBs (S1) | Kindly provided by Xinwen Chen (49) | Mouse | 1:50 (immunostaining) | |
| β-Actin | Cell Signaling Technology | 4970 | Rabbit | 1:10,000 (Western blot) |
| RNA polymerase II | Kindly provided by Kaiwei Liang (50) | Rabbit | 1:30 (HBVcircle ChIP-Seq) | |
| H3K27ac | Kindly provided by Kaiwei Liang | Rabbit | 1:20 (HBVcircle ChIP-Seq) | |
| H3K4me3 | Cell Signaling Technology | 9751S | Rabbit | 1:30 (HBVcircle ChIP-Seq) |
| H3K27me3 | Cell Signaling Technology | 9733S | Rabbit | 1:30 (HBVcircle ChIP-Seq) |
| HNF4α | Santa Cruz Biotechnology | sc-374229 | Mouse | 1:50 (ChIP assay) |
| RXRα | Cell Signaling Technology | 5388S | Rabbit | 1:50 (ChIP assay) |
| C/EBPα | Santa Cruz Biotechnology | sc-365318 X | Mouse | 1:50 (ChIP assay) |
| Rabbit IgG | Cell Signaling Technology | 2729 | Rabbit | 5 μg (ChIP assay) |
| Mouse IgG | Proteintech | B900620 | Mouse | 5 μg (ChIP assay) |