Figure 5.

Heat map representation of Trm signature genes. Clusters of differentially expressed transcripts within CD8⁺ IST Trm, MV Tem, and splenic Tm cell subset were ordered by K-means clustering analysis as described in Materials and Methods. Bulk RNAseq data derived from the three CD8⁺ memory T cell subsets were used for this analysis. (A) Differential expression of thirty-six previously reported Trm cell signature genes³ wherein log₂ fold change ≥1.5 with adjusted p ≤ 0.05 are shown. Blue side bar, splenic Tm cell-; brown side bar, lung MV Tem cell-; red sidebar, common MV Tem & lung IST Trm-; and maroon sidebar, IST Trm-specific gene expression pattern. (B) Differential expression analysis of transcriptional factors along with Trm signature genes^{3, 49} were performed as in (A) using bulk RNAseq data derived from the three memory T cell subsets. Highlighted are differentially expressed transcripts with log₂ fold change ≥1.2 with adjusted p ≤ 0.05 are shown. Sidebars are the same as in (A).