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. 2022 Nov 9;12:19101. doi: 10.1038/s41598-022-21620-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Heat map representation of pathway-specific gene expression changes. Clusters of differentially expressed transcripts specific within the indicated pathways (A–F) were ordered by K-means clustering analysis as described in Materials and Methods. Bulk RNAseq data derived from CD8⁺ IST Trm, MV Tem, and splenic Tm cell subsets were used for this analysis. (A) Chemokines and chemokine receptors. (B) Integrins. (C) Cell adhesion molecules. (D) Purinergic receptors. (E) inhibitory & activating killer cell lectin-like receptors. (F) T cell effector molecules.