Figure 7.

Prime boost vaccination with rOVA-3 without endotoxin depletion elicits a low but substantial level of B8R_70–78 and D1R_808–817-reactive CD8⁺ T cell response. (A) B0702^tg mice were primed and boosted by the i.n. route, two weeks apart with rOVA-3 mixed with αGC or with αGC alone. Lungs were collected d15 post boost. Cells were stained and gated on live CD8⁺ T cells and B8R_70–79, D1R_808-817 tetramer positive cells. Experiments were reproduced at least two times (n = 2 mice) and several times in our published report ⁴⁶. (B) B0702^tg mice were primed and boosted as in (A). Endotoxins were not depleted from this rOVA-3 preparation used for vaccination. On d15, splenic T cell response to the indicated B0702-restricited epitopes were determined by measuring IFN-γ response in an ELISpot assay. Data represent the mean of triplicate wells; representative of one experiment; (n = 3 mice). (C) The experiment in (B) was repeated with endotoxin-depleted rOVA-3 and the response measured in an IFN-γ specific ELISpot assay using the indicated peptides. Data represent the mean of triplicate wells; representative of two independent experiments; n = 3 mice in each experiment.