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. 2022 Nov 4;63(12):9. doi: 10.1167/iovs.63.12.9

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Figure 3. — PHLDA1 promotes cone and rod specification. (A) Trajectory analysis of the cone lineage showing progression from neurogenic RPC to photoreceptor precursors and cone. (B) Line chart showing the dynamic expression of PHLDA1, THRB, ARR3, and PDE6H. (C, D) The number of PHLDA1+ and BLIMP1+ cells were significantly downregulated in the shPHLDA1 (KD) group compared to the Scramble shRNA group. Scale bars = 20 µm. (E) Trajectory analysis of the rod lineage showing progression from neurogenic RPC to rod precursors and rod. (F) Line chart showing the dynamic expression of PHLDA1, NRL, NR2E3, and GNAT1. (G, H) The number of PHLDA1+, CRX+, and BLIMP1+ cells was significantly downregulated in the shPHLDA1 (KD) group compared to the Scramble shRNA group. Scale bars = 20 µm. Arrowheads indicate PHLDA1+ cells expressing scramble or PHLDA1 shRNA. (I, J) The number of PHLDA1+, CRX+, and BLIMP1+ cells was significantly increased in the PHLDA1 overexpression (OE) group compared to the Scramble. Scale bars = 20 µm. Arrowheads indicate the cells transfected by lentivirus (mCherry+). (K, L) The number of NRL+ and NR2E3+ cells was significantly downregulated in the shPHLDA1 (KD) group compared to the Scramble shRNA group. Scale bars = 20 µm. Arrowheads indicate NR2E3+ cells transfected by lentivirus (mCherry+). (M, N) The number of NRL+ and NR2E3+ cells was significantly increased in the PHLDA1 overexpression (OE) group compared to the Scramble group. Scale bars = 20 µm. Arrowheads indicate NR2E3+ cells transfected by lentivirus (mCherry+).