Figure 2.

A) Comparison of predicted (top) versus actual (bottom) confocal images of microgels after post-modification with tet–AFs at various mixed ratios. B) Photo of microgels after adding tet–AF. C) Tet–AF standard curve measured via fluorescence and confocal imaging. D) Fluorescence intensity of microgels after post-modification was measured and compared to predicted values. E) i,ii) Confocal images of various modified microgels show heterogeneity and precise control over post-modification via norbornene–tetrazine click reaction. iii) Photos of two different labeled MAP scaffolds adjacent to one another. F) Chemical schematic of attaching tetrazine to desired peptide via aminolysis. G) MALDI analysis of peptides after HPLC purification shows correct m/z of IKVAV and YIGSR tetrazine peptides.