Skip to main content
. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Cell Calcium. 2022 May 5;104:102594. doi: 10.1016/j.ceca.2022.102594

Fig. 1. Diversity and molecular basis of TPCs.

Fig. 1.

(A) Phylogeny tree of TPCs from different species. Amino acid seuqneces of TPC paralogues and orthologues from same and different species were retrived from NCBI protein database and then aligned and analyzed with methods of ClustalW and Minimum-Evolution Tree using program MEGA (v. 11) [66]. (B) Structure of open-state human TPC2 in complex with PI(3,5)P2 (pdb: 6NQ0) [37]. (C) Amino acid sequence alignment of selected TPCs of different species. Most amino acids are shaded collectively in colors according to their physicochemical properties (hydrophobic in yellow, polar in green, positively charged in blue and negatively charged in red) and special amino acids (glycine, histidine, tyrosine, and cysteine) are each shaded in other different colors. Residues discussed to be important for channel gating are shown in stick mode (sidechain only) in (B) and labeled according to human TPC2 sequence on top in (C).