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. 2022 Oct 19;11:e79940. doi: 10.7554/eLife.79940

Figure 6. mTOR-dependent oxidative phosphorylation is a key survival mechanism of human bone marrow stromal cell (hBMSC-CM)-mediated protection from FLT3 inhibition.

(A) MOLM-13 cells were treated with indicated drugs for 15 hr, followed by measurement of oxygen consumption rate (OCR) by Seahorse XF Cell Mito Stress Test (n = 5). Basal respiration, maximum respiration, and reserve respiratory capacity are shown at the bottom. (B) MOLM-13 cells were treated with indicated drugs for 15 hr, followed by luminescence-based ATP assays (normalizing relative light units [RLU] to cell number). (C) MOLM-13 cells were treated with 3 nM quizartinib and oligomycin A (Olig A) alone or together as indicated (increasing concentrations of Olig A of 1, 2, and 4 nM indicated by triangles) for 48 hr in RPMI or hBMSC-CM, followed by measurement of apoptosis (n = 3).

Figure 6.

Figure 6—figure supplement 1. Human bone marrow stromal cell (hBMSC-CM)-mediated partial restoration of oxidative phosphorylation is mTOR-dependent in MV4-11 cells.

Figure 6—figure supplement 1.

MV4-11 cells were treated with the indicated conditions for 15 hr, followed by measurement of oxygen consumption rate (OCR) by Seahorse XF Cell Mito Stress Test (n = 5). Basal respiration, maximum respiration, and reserve respiratory capacity are graphed. (B) MV4-11 cells were treated with the indicated conditions for 15 hr, followed by luminescence-based ATP assays (normalizing relative light units [RLU] to cell number) (n = 3).