FIG. 5.

Western blot showing specificities of affinity-isolated human antibodies against whole-bacterial-cell lysate of strain O35E. The antibodies were isolated as described in the text and diluted 1:1,000 for probing the Western blot. Lane A, antibodies isolated with UspA1; lane B, antibodies isolated with UspA2; lane C, antibodies isolated with the 74-kDa protein; lane D, starting serum pool. The mobilities and antigenic cross-reactivities of UspA1 and UspA2 were reported previously (21). The molecular mass markers (in kilodaltons) are indicated at left.