TABLE 3.
Absorbent | Bactericidal titer to the O35E strain in serum:
|
Bactericidal titer to the 1230-359 strain in serum:
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 1 | 2 | 3 | 4 | 5 | 6 | |
Noneb | 450 | 1,350 | 450 | 50 | 150 | 450 | 450 | 4,050 | 1,350 | 150 | 150 | 450 |
UspA1 | <50 | <50 | <50 | <50 | <50 | <50 | 50 | 150 | <50 | <50 | 50 | 150 |
UspA2 | <50 | 150 | <50 | <50 | <50 | <50 | 150 | 1,350 | 450 | <50 | 50 | 50 |
74-kDa protein | 450 | 1,350 | 450 | 50 | 50 | 150 | 450 | 4,050 | 1,350 | 150 | 150 | 450 |
Sera are those described in Table 2. The bactericidal titers to both the O35E and 1230-359 strains were determined with threefold dilutions of the sera starting at 1:50. The purified UspA1 and UspA2 proteins were prepared from the O35E strain.
Saline was added to samples in place of purified protein (at an equivalent volume), and samples were treated the same thereafter.