Figure 5:
The abscopal effect involved CD4+ and CD8+ T cells.
(A) Dual subcutaneous AE17-OVA+ tumor bearing mice were treated with four cycles of PBS, PLP (4 mg/kg), laser irradiation at 50 J/cm2, or PDT (PLP + laser). Mice were sacrificed on day 14 and spleens and right tumors were harvested for flow cytometry, while serum was collected for cytokine analysis. Summary of the percentage of CD3+ T cells, naive, central memory, and effector memory CD4+ T cells in the spleen of treated mice. (B) Summary of the percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, and effector memory CD8+ T cells in the non-irradiated, right tumors of treated mice. (C) Assessment of the concentration of interleukin-6, globulins, interleukin-2, and interferon-gamma in serum of mice (n = 5). Data are mean ± standard deviation. Statistical significance was determined using a one-way ANOVA, followed by a post hoc Tukey test. n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.