FIG. 1.

Dot immunoblot demonstrating chloroform extractability and isolation of CPS-500 from whole C. parvum. Shown are an excysted oocyst preparation before extraction (lanes 1 and 6), chloroform (lanes 2 and 7) and methanol (lanes 3 and 8) extracts, and chloroform extract following successive silicic acid chromatography (lanes 4 and 9) and HPLC (lanes 5 and 10) isolation steps. Lanes 1 through 5 were probed with MAb 18.44, and lanes 6 through 10 were probed with isotype control MAb. Serial twofold dilutions of each preparation are dotted from top to bottom.