FIG. 2.
Silver-stained SDS-PAGE gel of CPS-500 at successive stages of isolation from whole C. parvum, demonstrating relative freedom from protein and glycoprotein contamination. Shown are an excysted oocyst preparation (5 × 106 oocysts) prior to chloroform-methanol extraction (lane 2), the methanol-water-extracted phase (lane 4), and the chloroform-extracted phase before (lane 5) and after (lane 3) silicic acid chromatography. Lane 1 was loaded with sample buffer to identify silver stain artifacts.