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Dot immunoblot detection of the CPS-500 epitope recognized by MAb 18.44 before (lanes 1 and 5) and after treatment with 1 mM (lane 2), 5 mM (lane 3), or 10 mM (lane 4) periodate or with mixed glycosidases from T. cornutus (lane 7). Lane 6 (untreated CPS-500) and lane 8 (CPS-500 treated with mixed glycosidases) were probed with an isotype control MAb. Serial twofold dilutions of CPS-500 are dotted from top to bottom.
