TABLE 2.
Secretion of IL-8 and MCP-1 by HUVEC infected with C. trachomatis and C. pneumoniae
| Treatmenta | Concn (ng/ml)b
|
|
|---|---|---|
| IL-8 | MCP-1 | |
| Mock | 0.32 ± 0.11 | 0.3 ± 0.08 |
| C. trachomatis | ||
| Serovar L2 | 0.16 ± 0.05 | 0.3 ± 0.17 |
| Serovar A | 0.13 ± 0.02 | 0.28 ± 0.05 |
| Serovar E | 0.08 ± 0.04 | 0.28 ± 0.09 |
| C. pneumoniae TW-183 | 20.4 ± 0.9* | 5.0 ± 1.3* |
a
HUVEC monolayers were inoculated with C. trachomatis serovars L2, A, and E or C. pneumoniae TW-183 at a multiplicity of infection of 1:1. Mock-infected cells were treated with a suspension of lysed HEp-2 cells.
b
Levels of IL-8 and MCP-1 from culture supernatants were measured following 24 h of incubation by ELISA. Data indicate the means ± standard errors of the means of three separate experiments. In each experiment, duplicate wells were assayed separately for each condition for IL-8 and MCP-1 production. *, P < 0.001.