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Immunoblot of cell extracts of the parental strain, sigB mutant strain ALC1001, and complemented mutant strain ALC1497 probed with anti-sigB monoclonal antibody 2D7 (1:1,000 dilution). About 50 μg of cellular proteins was applied to each lane. The experiment was repeated two times, with essentially the same results. M. W., molecular weight. Numbers at left are in thousands.
