Table 3.
micro-CT, ashing and histomorphometrical analyses of lumbar vertebral body (L) and femur in Non-Orx and Orx rats either treated with enobosarm (EN), raloxifene (RAL) or combined treatment (EN + RAL)
| Parameters | Non-Orx | Orx | Orx + EN | Orx + RAL | Orx + EN + RAL | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |
| Micro-CT 3-D | ||||||||||
| L4 | ||||||||||
| B.BMD (g/cm3) | 0.85 | 0.02 | 0.86 | 0.02 | 0.88d | 0.04 | 0.84 | 0.03 | 0.88d | 0.04 |
| BV (mm3) | 64 | 7 | 491 | 5 | 59 | 8 | 59 | 7 | 70c,d | 7 |
| Ct.BMD (g/cm3) | 1.11 | 0.01 | 1.12 | 0.01 | 1.13 | 0.03 | 1.09a,b,c | 0.02 | 1.10c | 0.02 |
| Ct.V (mm3) | 23 | 3 | 19 | 3 | 24b | 4 | 20 | 4 | 291 | 6 |
| Tb.V (mm3) | 41 | 5 | 301 | 3 | 35 | 6 | 39 | 6 | 41 | 5 |
| St.V (mm3) | 65 | 11 | 63 | 7 | 60 | 11 | 51a,b | 7 | 49a,b,c | 7 |
| Femur | ||||||||||
| B.BMD (g/cm3) | 1.04 | 0.04 | 1.00 | 0.03 | 1.02 | 0.04 | 0.99a | 0.03 | 1.05b,d | 0.04 |
| BV (mm3) | 36 | 4 | 33 | 3 | 33 | 4 | 34 | 5 | 39b | 5 |
| Ct.BMD (g/cm3) | 1.25 | 0.04 | 1.23 | 0.03 | 1.25 | 0.03 | 1.25 | 0.03 | 1.26 | 0.03 |
| Ct.V (mm3) | 12 | 3 | 10 | 2 | 12 | 3 | 10 | 3 | 15bd | 4 |
| Tb.V (mm3) | 20 | 4 | 22 | 2 | 22 | 2 | 24 | 4 | 23 | 4 |
| St.V (mm3) | 14 | 3 | 17 | 2 | 14b | 3 | 16 | 2 | 12b,d | 2 |
| Ashing analysis | ||||||||||
| L4 | ||||||||||
| Anorganic content (%) | 28.4 | 1.5 | 25.4a | 3.5 | 27.3 | 2.6 | 28.0 | 3.0 | 30.0b | 2.1 |
| Organic content (%) | 71.6 | 1.5 | 74.6a | 3.5 | 72.7 | 2.6 | 72.2 | 3.0 | 70.3b | 2.1 |
| Mg+ (%) | 0.68 | 0.02 | 0.66 | 0.01 | 0.70b,d | 0.02 | 0.66 | 0.02 | 0.67 | 0.02 |
| Ca2+/PO43− | 1.54 | 0.04 | 1.51 | 0.03 | 1.62b | 0.13 | 1.71a,b | 0.04 | 1.72a,b,c | 0.03 |
| Femur | ||||||||||
| Anorganic content (%) | 43.3 | 2.4 | 40.3a | 2.1 | 43.9b | 2.0 | 43.8b | 2.1 | 47.31 | 3.2 |
| Organic content (%) | 56.8 | 2.4 | 60.0a | 2.1 | 56.1b | 2.0 | 56.3b | 2.1 | 52.71 | 3.2 |
| Mg+ (%) | 0.66 | 0.02 | 0.66 | 0.01 | 0.67 | 0.01 | 0.65 | 0.02 | 0.65 | 0.02 |
| Ca2+/PO43− | 1.26 | 0.05 | 1.25 | 0.04 | 1.25 | 0.03 | 1.25 | 0.04 | 1.29 | 0.05 |
| Histomorphometry, L5 | ||||||||||
| Ob/B.Pm (N/mm) | 57 | 13 | 60 | 12 | 71 | 14 | 69 | 15 | 66 | 23 |
| Oc/B.Pm (N/mm) | 2.8 | 0.6 | 3.3 | 0.9 | 3.2 | 1.3 | 2.8 | 1.0 | 2.8 | 0.9 |
| Ot/B.Ar (N/mm2) | 565 | 159 | 589 | 176 | 585 | 85 | 536 | 201 | 510 | 89 |
1differs from all other groups, a-differs from Non-Ovx, b- differs from Ovx, d- differs from OS-0.4 (p < 0.05, Dunn’s test: Femur BV, Femur Ct.BMD, Femur St.V, Ob/B.Pm, and Oc/B.Pm; Tukey test: all other data)
B Bone, Ct cortical bone, Tb trabecular bone, St soft tissue, BMD bone mineral density, V volume