FIG. 10.

Effects of STF on antigen uptake by Mφ. PBMC were incubated overnight in the absence (media) or presence of STF (4 μg/ml), TT (2 μg/ml), or LPS (10 ng/ml), and the uptake of FITC-labeled dextran was determined by flow cytometry, as described in Materials and Methods. The percentage of FITC-dextran-positive cells was calculated from cells gated on the Mφ region, characterized by high forward light scatter versus high side light scatter. Shown are the means (standard deviations [SD]) of the percentages of FITC-dextran-positive cells observed with PBMC from three volunteers (two vaccinated, one unvaccinated). The P value is as compared to cells treated with medium or TT.