Skip to main content
. 2022 Oct 27;13:986565. doi: 10.3389/fendo.2022.986565

Figure 2.

Figure 2

Classical and non-classical inflammasome assembly. The canonical pathway is mediated by caspase-1. When NLRP1, NLRC4, NLRP3, AIM, and Pyrin inflammatory sensors receive different stimuli, recruiting ASC and caspase-1 to mediate the assembly of inflammasomes and activate caspase-1. Among them, the assembly of NLRP3 and NLRC4 inflammasomes requires the participation of NEK7 and NAIPs, respectively, and NLRP1 and NLRC4 can directly activate case page-1 due to their CARD domains. After activation of Caspase-1, GSDMD and pro-IL-1β/18 is cleaved, and the pore formed by GSDMD on the cell membrane surface mediates secretion of IL-1β/18 out of the cell and induces pyroptosis. The non-canonical pathway is mediated by caspase-4/5/11; LPS of Gram-negative bacteria directly induces caspase-4/5/11 activation and cleaves GSDMD and Pannexin-1. N-GSDMD induces caspase-1 activation by activating NLRP3 inflammasome assembly, which mediates the release of inflammatory mediators and pyroptosis.