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Vertical view of Aspergillus niger biofilm grown on a glass coupon center portion (top) and corner portion (bottom) stained with SYTO9 and PI. (A & D) Red channel - Inactive/Dead cells with PI (B & E) Green channel-Living/active cells with SYTO9. (C & F) Overlay of both active/live (SYTO9) and Inactive/dead (PI) cells. Scale bars = 200 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
