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. 2022 Nov 9;13(11):942. doi: 10.1038/s41419-022-05393-5

Fig. 3. HOXA11-AS can mediate its own transcription via regulating let-7b-5p/CTHRC1/β-catenin/c-Myc axis.

Fig. 3

A Venn diagram showing the predicted target genes of let-7b-5p. B U87 and LN229 cells were co-transfected with reporter vectors containing the wild-type CTHRC1 3′UTR (CTHRC1 WT) or CTHRC1 3′UTR with a mutated let-7b-5p binding site (CTHRC1 Mut) and either let-7b-5p mimics or scramble oligonucleotide (NC-mimics). C The effect of HOXA11-AS knockdown on β-catenin transcriptional activity was detected by TOP/FOP assay. D HOXA11-AS regulated the transcriptional activity of β-catenin depending on CTHRC1 via TOP/FOP assay. E HOXA11-AS regulating the transcriptional activity of c-Myc depending on CTHRC1 was demonstrated via luciferase assay. F HOXA11-AS regulating the transcriptional activity of c-Myc depending on let-7b-5p was verified via luciferase assay. G The binding sites of c-Myc bound to the HOXA11-AS promoter region were verified via ChIP assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.