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. 2022 Nov 9;13(11):942. doi: 10.1038/s41419-022-05393-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The overall workflow of integrated analysis of transcriptomics and proteomics. B Scatterplot of GO items in both transcriptomic and proteomic data sets. C Scatterplot of KEGG items in both transcriptomic and proteomic data sets. The red plots indicated these items significant enrichment of both omics; the green plots indicated these items enrichment of single omics; the black plots indicated these items were not enriched in both omics. The expression levels of 17 DEGs enriched in the TNF-α pathway from transcriptomics were verified by RT-qPCR in U87 (D) and LN229 (E) cells. Each data point was calculated from averages of biological triplicates. Key proteins in TNF-α pathway were validated by western blot in U87 (F) and LN229 (G) cells infected with Lv-HOXA11-AS. GAPDH was used as an internal control. Xenograft mouse models were generated by subcutaneous injection of U87 cells into nude mice. Seven days after implanted, mice were treated with si-NC (n = 10) or si-Tpl2 (n = 10). Representative image of tumor dissected tumors (H), growth curve (I) and tumor weight (J) in si-NC or si-Tpl2-treated xenograft mouse models. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.