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. 2022 Nov 9;13(11):942. doi: 10.1038/s41419-022-05393-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Effect of HOXA11-AS on Tpl2 mRNA stability was confirmed via ActD assay. B Effect of HOXA11-AS on Tpl2 transcriptional activity was verified by luciferase assay. C The combination of HOXA11-AS and c-Jun was verified by RIP experiment. D The binding of HOXA11-AS to four different domains of c-Jun (Jun-FL, Jun-TAD, Jun-241 and Jun-DBD) was verified by luciferase assay in U87 and LN229 cells. E The binding sites of c-Jun to the Tpl2 promoter region were verified by ChIP assay. F Luciferase assay was used to determine the region of the Tpl2 promoter that might interact with c-Jun. G Luciferase assay was used to determine the region of the Tpl2 promoter that might interact with HOXA11-AS. H C-Jun binding to Tpl2 promoter region depending on HOXA11-AS was determined by ChIP assay. I, J HOXA11-AS regulating Tpl2 expression depending on c-Jun was confirmed via RT-qPCR and western blot. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.