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. 2022 Nov 9;13:6762. doi: 10.1038/s41467-022-34434-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The relative mRNA m⁶A level infected with METTL14 overexpression in NRCM (n = 6/group) or mouse hearts (n = 4 /group). b Crystal structure of METTL3–METTL14 complex (PDB ID: 5IL0) showing the positively charged groove formed by METTL3-METTL14. Charged residues which are contributed by METTL14 are labeled as sticks. c The relative mRNA m⁶A level in hearts treated as indicated (n = 4/group). d Representative western blot and statistical data of METTL14 expression levels in mouse hearts treated as indicated (n = 6 mice/group). e Cardiac gross morphology, HW, and HW/TL in mouse hearts treated as indicated (n = 12 mice/group). f Representative images of WGA staining and quantification of the relative cell cross-sectional area in the mouse hearts treated as indicated (n = 6 mice/group). Scale bar: 20 μm. g Representative images of immunofluorescent staining and quantification of cardiomyocytes EdU positive ratio in the mouse hearts treated as indicated (n = 6 mice/group). Scale bar: 20 μm. h Representative images of immunofluorescent staining and quantification of the cardiomyocytes Ki67 positive ratio in the mouse hearts treated as indicated (n = 6 mice/group). Scale bar: 20 μm. NRCM, neonatal rat cardiomyocyte; Fugw, control without METTL14 overexpression; METTL14 OE, METTL14 overexpression; AAV9-cTnT-ctrl, cardiac-specific troponin-T promoter-driven control AAV9; AAV9-cTnT-METTL14, cardiac-specific troponin-T promoter-driven METTL14 overexpression AAV9; AAV9-cTnT-WT^METTL14, cardiac-specific troponin-T promoter-driven wild-type METTL14 overexpression AAV9; AAV9-cTnT- Mut^METTL14, cardiac-specific troponin-T promoter-driven METTL14 R254/R255A-R298P-D312A mutant overexpression AAV9; HW, heart weight. HW/TL, heart weight/tibia length. WGA, Wheat Germ Agglutinin. EdU, 5-ethynyl-2’-deoxyuridine. All data are expressed as means ± SD. a Independent-sample t-test, two-sided; c one-way ANOVA followed by Dunnett T3 test; d–h two-way ANOVA followed by Tukey’s post hoc test. Source data are provided as a Source Data file.