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. 2022 Nov 9;13:6762. doi: 10.1038/s41467-022-34434-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schedule of virus injection and acute myocardial I/R injury model establishment. b Representative western blot and statistical data of METTL14 expression levels in mouse hearts treated as indicated (n = 6 mice/group). c Representative images of 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantification of the area at risk/left ventricular weight (AAR/LV) ratio and the infarct size/area at risk (INF/AAR) ratio (n = 7 and 8 mice, respectively). d Representative images of immunofluorescence staining and quantification of the TUNEL positive cardiomyocytes in mouse hearts treated as indicated (n = 6 mice/group). Scale bar: 20 μm. e Representative western blot and statistical data of myocardium apoptosis by detection of Bax, Bcl2, caspase 3, and Cleaved-Caspase 3 expression levels in mouse hearts treated as indicated (n = 6 mice/group). AAV9-cTnT-shScr, cardiac-specific troponin-T promoter-driven Scramble AAV9; AAV9-cTnT-shMETTL14, cardiac-specific troponin-T promoter-driven METTL14 knockdown AAV9; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; ns, nonstatistically significant; I/R, ischemia-reperfusion. All data are expressed as means ± SD. b, d, and e, two-way ANOVA followed by Tukey’s post hoc test; c, independent-sample t-test, two-sided. Source data are provided as a Source Data file.